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3. What is the resolving power of unaided/naked human eye? A. 0,2 micrometer B. 200 nanometer C. 200 milimeter D. 0,2 milimeter E. 2000 micrometer 14. Reticular fibers are stained with __ A. Masson's trichrome B. Silver salts C. Orcein D. Periodic acid-Schiff E. Safranin 15. Excretion of spermium into the seminiferous tubule is a type of A. Autocrine secretion B. Apocrine secretion C. Merocrine secretion D. Holocrine secretion E. Paracrin secretion 16. Which of the following is false about connective tissue? A. Loose connective tissue contains faintly stained ground substance with fine fibers of collagen. B. Reticular connective tissue contains type III collagen. C. Mucoid tissue is an embryonic type of connective tissue. D. Dense irregular connective tissue forms the capsule of testis. E. Wharton's jelly is an exampele of dense regular connective tissue. 17. Which of the following is an anchoring collagen? A. Type II collagen B. Type III collagen C. Type IV collagen D. Type VII collagen E. Type I collagen 18. What is the difference between basal lamina and basement membrane? 19. What are the steps of tissue preparation for light microscopy? 20. Why do we call pseudostratified columnar epithelium as pseudostrafied?
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3. The resolving power of the unaided/naked human eye is approximately 0.2 millimeters (D).14. Reticular fibers are stained with silver salts (B).15. Excretion of spermium into the seminiferous tubule is a type of merocrine secretion (C).16. The false statement about connective tissue is that Wharton's jelly is an example of dense regular connective tissue (E).17. Type I collagen is an anchoring collagen (E).18. The difference between basal lamina and basement membrane is that the basal lamina is a layer of extracellular matrix secreted by the epithelial cells, while the basement membrane is a thicker layer that includes the basal lamina and additional components such as reticular fibers.19. The steps of tissue preparation for light microscopy are: 1. Fixation: The tissue is preserved by stopping cellular processes and maintaining its structure. 2. Dehydration: The tissue is dehydrated using a series of alcohol or acetone solutions. 3. Clearing: The dehydrated tissue is cleared using a solvent such as xylene or benzene. 4. Embedding: The cleared tissue is embedded in a solid medium, such as paraffin wax or resin, to provide support and facilitate thin sectioning. 5. Sectioning: Thin slices of the embedded tissue are cut using a microtome. 6. Staining: The tissue sections are stained using specific dyes to enhance contrast and highlight different cellular components.20. Pseudostratified columnar epithelium is called "pseudostratified" because it appears to have multiple layers of cells, but in reality, all cells are in contact with the basement membrane. The term "pseudo" means false, indicating that the appearance of multiple layers is deceptive.