5. During the preparation of tissue sample a routine H&E slide , what allows the tissue to be visualized? A. Fixation B. Embedding in paraffin C. Staining D. Slicing

The correct answer is C. Staining. Staining is the process that allows the tissue to be visualized during the preparation of a tissue sample. Stains, such as Hematoxylin and Eosin (H&E), are used to color the tissue, making it easier to see the different structures and components under a microscope. Fixation, embedding in paraffin, and slicing are also important steps in tissue sample preparation, but they do not directly allow the tissue to be visualized.