1. What is the magnification of the oil immersion lens when utilizing a microscope? A. 4X B. 10X C. 40X D. 100X 2. Which of the following stain pink with H&E stain? A. Cytoplasm B. Collagen fibers C. Nucleus D. Elastic tibers 3. During the preparation of tissue sample a routine HÉE slide, what step occurs after the tissue is fixated? A. Embedding in paraffin B. Staining C. Slicing D. Observation 4. What are Sudan stains used primarily for? A. Blood B. Lipids C. Neryous tissue D. Elastic tibers

1. The correct answer is D. 100X. The magnification of the oil immersion lens when utilizing a microscope is typically 100X. This lens is used for observing specimens at the highest magnification, allowing for detailed and precise observations.<br /><br />2. The correct answer is C. Nucleus. In H&E staining, the nucleus stains pink due to the affinity of hematoxylin for DNA, while the cytoplasm stains red or pink due to the affinity of eosin for proteins.<br /><br />3. The correct answer is A. Embedding in paraffin. After the tissue is fixated, the next step in the preparation of a routine H&E slide is embedding in paraffin. This process involves infiltrating the tissue with paraffin wax to create a solid block that can be sectioned for microscopic examination.<br /><br />4. The correct answer is B. Lipids. Sudan stains are primarily used for staining lipids. These stains, such as Sudan IV or Sudan Black, selectively stain lipid-containing structures, making them visible under a microscope. This is particularly useful in studies related to lipid metabolism and the identification of lipid deposits in tissues.